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Lipid Rafts : Methods and Protocols (1st ed. 2021)

By: Bieberich, Erhard(Edited by)

Part of the Methods in Molecular Biology series
1071608134 / 9781071608135
Hardback
28/08/2020
Published 28/08/2020
United States
178 x 254 mm 350 pages, 62 Illustrations, color; 17 Illustrations, black and white; XI, 350 p. 79 illus., 62 illu
Professional & Vocational  Learn More

This volume gives a comprehensive insight into established and novel methods to analyze the structure and function of lipid rafts.

This book covers topics such as isolation of lipid rafts and their functional analysis using biochemical methods; visualization of lipid rafts and their interaction with proteins using fluorescence-related methods; preparation of giant lipid vesicles and fluorescence spectroscopy; FRET and FRAP; and using photo-activated cross-linking of a ceramide analog combined with proximity ligation assay.

Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Lipid Rafts: Methods and Protocols is a valuable resource for both novice and expert researchers interested in learning more about the function of lipid rafts in many areas of cell biology and medicine.

BIC:

PNFS Spectrum analysis, spectrochemistry, mass spectrometry, PSBH Lipids

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