Transport in Plants II : Part A Cells (Softcover reprint of the original 1st ed. 1976)

As plant physiology increased steadily in the latter half of the 19th century, problems of absorption and transport of water and of mineral nutrients and problems of the passage of metabolites from one cell to another were investigated, especially in Germany.

JUSTUS VON LIEBIG, who was born in Darmstadt in 1803, founded agricultural chemistry and developed the techniques of mineral nutrition in agricul­ ture during the 70 years of his life.

The discovery of plasmolysis by NAGEL! (1851), the investigation of permeability problems of artificial membranes by TRAUBE (1867) and the classical work on osmosis by PFEFFER (1877) laid the foundations for our understanding of soluble substances and osmosis in cell growth and cell mechanisms.

Since living membranes were responsible for controlling both water movement and the substances in solution, "permeability" became a major topic for investigation and speculation.

The problems then discussed under that heading included passive permeation by diffusion, Donnan equilibrium adjustments, active transport processes and antagonism between ions.

In that era, when organelle isolation by differential centrifugation was unknown and the electron microscope had not been invented, the number of cell membranes, their thickness and their composition, were matters for conjecture.

The nature of cell surface membranes was deduced with remarkable accuracy from the reactions of cells to substances in solution.

In 1895, OVERTON, in U. S. A. , published the hypothesis that membranes were probably lipid in nature because of the greater penetration by substances with higher fat solubility.