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DNA Modifications : Methods and Protocols (1st ed. 2020)

By: Gering, Martin(Edited by) Ruzov, Alexey(Edited by)

Part of the Methods in Molecular Biology series
1071608754 / 9781071608753
Published 14/09/2020
United States
178 x 254 mm 522 pages, 70 Illustrations, color; 27 Illustrations, black and white; IV, 522 p. 97 illus., 70 illu
Professional & Vocational  Learn More

This book provides an overview of methods and experimental protocols that are currently used to analyze the presence and abundance of non-canonical DNA nucleotides in different biological systems.

Focusing particularly on the newly discovered and less studied DNA modifications that are enzymatically produced and are likely to play specific roles in various biological processes, the volume explores chromatography- and mass spectrometry-based techniques for the detection and quantification of DNA modifications, antibody-based approaches to study their spatial distribution in different cells and tissues, and methods to analyze their genomic distribution with the help of bioinformatics tools that interrogate the corresponding datasets.

Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and comprehensive, DNA Modifications: Methods and Protocols serves as an ideal guide to research scientists and PhD students in this rapidly developing discipline, and, thus, will ultimately contribute to deciphering the roles of non-canonical DNA nucleotides in different biological systems.


PS Biology, life sciences, PSAK Genetics (non-medical)

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