Guide to Yeast Genetics and Molecular Biology - Volume 194

"Guide to Yeast Genetics and Molecular Biology" presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology.

Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast.

It covers: basic methods in yeast genetics; physical and genetic mapping; making and recovering mutants; cloning and Recombinant DNA methods; high-efficiency transformation; preparation of yeast artificial chromosome vectors; basic methods of cell biology; immunomicroscopy; protein targeting assays; biochemistry of gene expression; vectors for regulated expression; and, isolation of labeled and unlabeled DNA, RNA, and protein.