Lipid Modifications of Proteins

General Description of the Volume: This volume presents the best techniques and strategies for the study of lipid modified proteins, with particular emphasis on methods which evaluate the functional significance or biological impact of lipid modification.

Included are the biological methods for the study of function (yeast genetics; cloning strategies; mutational analysis; expression systems), biochemical methods for the study and purification of enzymes or modified proteins (in vitro assays using peptide, native, or recombinant protein substrates; coupled in vitro cDNA transcription, translation/modification; bacolovirus expression; lipid analogs/inhibitors); physical methods for the identification of lipid groups (cleavage techniques; modification techniques; simple separations: TLC, GC, HPLC, ES/MS, tandem MS).

General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry.

Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.