Techniques for the Analysis of Complex Genomes

Faced with the search for genes associated with human diseases and the prospect of mapping and sequencing the human genome and those of other organisms, scientists must employ new strategies designed to map and clone large regions of DNA.

Written by internationally renowned scientists, and containing contributions from some of the leading laboratories in the world, Techniques for the Analysis of Complex Genomes describes genomic mapping by pulsed field gel electrophoresis, and provides a detailed account of the cloning of large fragments of DNA using yeast artificial chromosomes (YACs) and bacteriophage PI cloning systems.